Product Description

Gelin-S® is thiol-modified gelatin (denatured collagen) and is a component of the HyStem®-C, and HyStem-HP hydrogel kits. Most cells do not grow well on Gelin-S–only hydrogels. Instead, Gelin-S should be used in conjunction with Glycosil® (thiol-modified hyaluronic acid) or Heprasil® (thiol-modified hyaluronic acid with thiol-modified heparin). Reconstituted Gelin-S remains liquid at 15 to 37°C.

The gelatin used to make Gelin-S is from Type A Gelatin, Bloom 250, derived from porcine skin.

Gelin-S® (thiol-modified gelatin) is packaged in 5.0 mL vials containing 50mg. Vials are blanketed by nitrogen and under a slight vacuum.

Store Gelin-S in the original vial, unopened, at -20 °C for up to one year. Do not uncap the Gelin-S vials since they will crosslink in the presence of oxygen. Use a syringe and needle to add DG Water to the vials.

Note: It is recommended to reconstitute each vial in its entirety.

Directions for Use

Gelin-S is prepared by dissolving the lyophilized solid in the DG Water (or any sterile, degassed, deionized water). When reconstituted, it will be in 1x phosphate buffered saline (PBS), pH ~7.4. The amount of DG Water used for dissolution depends on the vial.

Gelin-S should be prepared in the following manner:

  1. Allow the Gelin-S vial to come to room temperature.
  2. Under aseptic conditions, using a syringe and needle, add 5 mL of DG Water to the Gelin-S vial.
  3. Place the vial horizontally on a rocker or shaker. It will take <40 minutes for the solids to fully dissolve. Warming to 37°C or less and/or gently vortexing will speed up dissolving time. Solutions will be clear and slightly viscous.
  4. Gelin-S will not form a hydrogel even if Extralink® is added. To form a hydrogel, it must be mixed with Extralink and Glycosil®, or Heprasil®.

Product References

References for HyStem®:

Gaetani, R., et al. (2015) Epicardial application of cardiac progenitor cells in a 3D-printed gelatin/hyaluronic acid patch preserves cardiac function after myocardial infarction. Biomaterials 61: 339-348. PMID: 17335875.

Prestwich, G.D., et al. (2007) 3-D culture in synthetic extracellular matrices: new tissue models for drug toxicology and cancer drug discovery. Adv Enzyme Regul 47: 196-207. PMID: 17335875.

Shu, X.Z., et al. (2006) Synthesis and evaluation of injectable, in situ crosslinkable synthetic extracellular matrices for tissue engineering. J Biomed Mater Res A 79: 901-912. PMID: 16941590.

Shu, X.Z., et al. (2003) Disulfide-crosslinked hyaluronan-gelatin hydrogel films: a covalent mimic of the extracellular matrix for in vitro cell growth. Biomaterials 24: 3825-3834. PMID: 12818555.

S. Cai, et al. (2005) Injectable glycosaminoglycan hydrogels for controlled release of human basic fibroblast growth factor.Biomaterials, 26, 6054-6067.

D. B. Pike, et al. (2006) Heparin-regulated release of growth factors in vitro and angiogenic response in vivo to implanted hyaluronan hydrogels containing VEGF and bFGF. Biomaterials, 27, 5242–5251.

G. D. Prestwich, et al. (2007) 3-D Culture in Synthetic Extracellular Matrices: New Tissue Models for Drug Toxicology and Cancer Drug Discovery. invited, Adv. Enz. Res., in press (2007).

X. Z. Shu, et al, (2006) Synthesis and Evaluation of Injectable, In Situ Crosslinkable Synthetic Extracellular Matrices (sECMs) for Tissue Engineering. J. Biomed Mater. Res. A, 79A(4), 901-912.

Shu, X.Z., et al. (2004) In situ crosslinkable hyaluronan hydrogels for tissue engineering. Biomaterials 25: 1339-1348. PMID: 14643608. 

Mehra, T.D., et al. (2006) Molecular stenting with a crosslinked hyaluronan derivative inhibits collagen gel contraction. J Invest Dermatol 126: 2202-2209. PMID: 16741511. 

Shu, X.Z., et al. (2004) Attachment and spreading of fibroblasts on an RGD peptide-modified injectable hyaluronan hydrogel. J Biomed Mater Res A 68: 365-375. PMID: 14704979. 

Ghosh, K., et al. (2007) Cell adaptation to a physiologically relevant ECM mimic with different viscoelastic properties. Biomaterials 28: 671-679. PMID: 17049594.

Product Certificate of Analysis

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Product Disclaimer

This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.